ANOVA-Like Differential Expression (ALDEx) Analysis for Mixed Population RNA-Seq
نویسندگان
چکیده
منابع مشابه
ANOVA-Like Differential Expression (ALDEx) Analysis for Mixed Population RNA-Seq
Experimental variance is a major challenge when dealing with high-throughput sequencing data. This variance has several sources: sampling replication, technical replication, variability within biological conditions, and variability between biological conditions. The high per-sample cost of RNA-Seq often precludes the large number of experiments needed to partition observed variance into these c...
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RNA-Seq is increasingly being used for gene expression profiling. In this approach, next-generation sequencing (NGS) platforms are used for sequencing. Due to highly parallel nature, millions of reads are generated in a short time and at low cost. Therefore analysis of the data is a major challenge and development of statistical and computational methods is essential for drawing meaningful conc...
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Background Differential expression analysis of RNA sequencing (RNA-seq) data typically relies on reconstructing transcripts or counting reads that overlap known gene structures. Previously we introduced an intermediate approach called differentially expressed region (DER) finder that seeks to identify contiguous regions of the genome showing differential expression signal at single base resolut...
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Fundamentally, many high throughput sequencing approaches generate similar data: reads are mapped to features in each sample, these features are normalized, then statistical difference between the features composing each group or condition is calculated. The standard statistical tools used to analyze RNA-seq, ChIP-seq, 16S rRNA gene sequencing, metagenomics, etc. are fundamentally different for...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2013
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0067019